| 質粒名稱: | pMK-N. tabacum trnI/trnA |
|---|---|
| 出品公司: | 愛迪基因 |
| 目錄編號: | 123579 |
| 存儲實驗室: | Scott Lenaghan, C. Neal Stewart |
|
載體骨架基本信息 |
|
| 載體名稱: | pMK-RQ |
| 載體出品公司: | -- |
| 載體抗性: | 卡那霉素 |
| 空載體大小: | -- |
| 完整質粒大小: | -- |
| 載體修飾: | -- |
| 載體類型: | 植物表達；葉綠體轉化載體 |
| 篩選標記: | -- |
| 高拷貝/低拷貝: | 高拷貝 |
| 生長條件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名稱: | N. tabacum trnI/trnA integration site |
| 物種來源: | -- |
| 插入大小: | -- |
| 基因突變: | -- |
| GenBank編號 | -- |
| 啟動子 | -- |
| 標簽/融合蛋白: | -- |
| 克隆方法: | 限制性內切酶 |
| 5' 克隆位點: | -- |
| 3' 克隆位點: | -- |
| 5' 測序引物 | -- |
| 3' 測序引物 | -- |
|
文獻引用方法 |
|
| 材料和方法部分: | pMK-N. tabacum trnI/trnA was a gift from Scott Lenaghan & C. Neal Stewart (Addgene plasmid # 123579 ; //n2t.net/addgene:123579 ; RRID:Addgene_123579) |
| 參考文獻部分: | MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology. Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S. Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. 10.1104/pp.18.01220 PubMed 30679266 |
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pMK-N. tabacum trnI/trnA載體質粒基本信息
pMK-N. tabacum trnI/trnA質粒圖譜載體圖譜和pMK-N. tabacum trnI/trnA載體序列質粒序列多克隆位點信息
pMK-N. tabacum trnI/trnA質粒載體簡介
pMK-N. tabacum trnI/trnA質粒序列載體序列
LOCUS pMK-N. tabacum trnI/trnA 5116 bp ds-DNA circular SYN 27-JUN-2019
DEFINITION MoChlo kit.
ACCESSION .
VERSION .
KEYWORDS pMK-N. tabacum trnI/trnA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5116)
AUTHORS Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN,
Lenaghan S
TITLE MoChlo: A versatile modular cloning toolbox for chloroplast
biotechnology.
JOURNAL Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi:
10.1104/pp.18.01220.
PUBMED 30679266
REFERENCE 2 (bases 1 to 5116)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 27, 2019 from SnapGene Server 1.1.58
//www.snapgene.com
FEATURES Location/Qualifiers
source 1..5116
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 278..300
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 292..309
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 293..309
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 320..339
/label=T7
/note="T7 promoter, forward primer"
promoter 320..338
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 343..354
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
CDS 1638..2357
/codon_start=1
/product="brightly fluorescent monomeric variant of GFP"
/label=mEmerald
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(1683..1704)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1944..1963)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 2291..2312
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(3322..3339)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3432..4020)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3512..3531)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4194..4988)
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
promoter complement(4989..5093)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
361 aggccgcatg ggccttgtac acaccgcccg tcacactatg ggagctggcc atgcccgaag
421 tcgttacctt aaccgcaagg agggggatgc cgaaggcagg gctagtgact ggagtgaagt
481 cgtaacaagg tagccgtact ggaaggtgcg gctggatcac ctccttttca gggagagcta
541 atgcttgttg ggtattttgg tttgacactg cttcacaccc ccaaaaaaaa gaagggagct
601 acgtctgagt taaacttgga gatggaagtc atctttcctt tctcgacggt gaagtaagac
661 caagctcatg agcttattat cctaggtcgg aacaagttga taggaccccc ttttttacgt
721 ccccatgttc cccccgtgtg gcgacatggg ggcgaaaaaa ggaaagagag ggatggggtt
781 tctctcgctt ttggcatagc gggcccccag tgggaggctc gcacgacggg ctattagctc
841 agtggtagag cgcgcccctg ataattgcgt cgttgtgcct gggctgtgag ggctctcagc
901 cacatggata gttcaatgtg ctcatcggcg cctgaccctg agatgtggat catccaaggc
961 acattagcat ggcgtactcc tcctgttcga accggggttt gaaaccaaac tcctcctcag
1021 gaggatagat ggggcgattc gggtgagatc caatgtagat ccaactttcg attcactcgt
1081 gggatccggg cggtccgggg gggaccacca cggctcctct cttctcgaga atccatacat
1141 cccttatcag tgtatggaca gctatctctc gagcacaggt ttagcaatgg gaaaataaaa
1201 tggagcacct aacaacgcat cttcacagac caagaactac gagatcgccc ctttcattct
1261 ggggtgacgg agggatcgta ccattcgagc cgtttttttc ttgactcgaa atgggagcag
1321 gtttgaaaaa ggatcttaga gtgtctaggg ttgggccagg aggctctctt aacgccttct
1381 tttttcttct catcggagtt atttcacaaa gacttgccag ggtaaggaag aaggggggaa
1441 caagcacact tggagagcgc agtacaacgg agagttgtat gctgcgttcg ggaaggatga
1501 atcgctcccg aaaaggaatc tattgattct ctcccaattg gttggaccgt aggtgcgatg
1561 atttacttca cgggcgagct ctctggttca agtccaggat ggcccagctg cgaattcgtt
1621 taaactgcct tgtcttcatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
1681 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
1741 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
1801 ccgtgccctg gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc ttcgcccgct
1861 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
1921 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
1981 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
2041 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaaggtc tatatcaccg
2101 ccgacaagca gaagaacggc atcaaggtga acttcaagac ccgccacaac atcgaggacg
2161 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
2221 tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga
2281 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
2341 acgagctgta caagtaagaa gacaagggag tttaaacaag cttctccact tggctcgggg
2401 ggatatagct cagttggtag agctccgctc ttgcaattgg gtcgttgcga ttacgggttg
2461 gatgtctaat tgtccaggcg gtaatgatag tatcttgtac ctgaaccggt ggctcacttt
2521 ttctaagtaa tggggaagag gaccgaaacg tgccactgaa agactctact gagacaaaga
2581 tgggctgtca agaacgtaga ggaggtagga tgggcagttg gtcagatcta gtatggatcg
2641 tacatggacg gtagttggag tcggcggctc tcccagggtt ccctcatctg agatctctgg
2701 ggaagaggat caagttggcc cttgcgaaca gcttgatgca ctatctccct tcaacccttt
2761 gagcgaaatg cggcaaaaga aaaggaagga aaatccatgg accgacccca tcatctccac
2821 cccgtaggaa ctacgagatc accccaagga cgccttcggc atccaggggt cacggaccga
2881 ccatagaacc ctgttcaata agtggaacgc attagctgtc cgctctcagg ttgggcagtc
2941 agggtcggag aagggcaatg actcattctt agttagaatg ggattccaac tcagcacctt
3001 ttgagtgaga ttttgagaag agttgctctt tggagagcac agtacgatga aagttgtaag
3061 ctgtgttcgg gggggagtta ttgtctatcg ttggcctcta tggtagaatc agtcggggga
3121 cctgagaggc ggtggtttac cctgcggcgg atgtcagcgg ttcgagtccg cttatctcca
3181 actcgtgaac ttagccgata caaagctctg ggcctcatgg gccttccttt cactgcccgc
3241 tttccagtcg ggaaacctgt cgtgccagct gcattaacat ggtcatagct gtttccttgc
3301 gtattgggcg ctctccgctt cctcgctcac tgactcgctg cgctcggtcg ttcgggtaaa
3361 gcctggggtg cctaatgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
3421 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
3481 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
3541 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
3601 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
3661 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
3721 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
3781 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
3841 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
3901 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
3961 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
4021 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
4081 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
4141 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttattagaaa
4201 aattcatcca gcagacgata aaacgcaata cgctggctat ccggtgccgc aatgccatac
4261 agcaccagaa aacgatccgc ccattcgccg cccagttctt ccgcaatatc acgggtggcc
4321 agcgcaatat cctgataacg atccgccacg cccagacggc cgcaatcaat aaagccgcta
4381 aaacggccat tttccaccat aatgttcggc aggcacgcat caccatgggt caccaccaga
4441 tcttcgccat ccggcatgct cgctttcaga cgcgcaaaca gctctgccgg tgccaggccc
4501 tgatgttctt catccagatc atcctgatcc accaggcccg cttccatacg ggtacgcgca
4561 cgttcaatac gatgtttcgc ctgatgatca aacggacagg tcgccgggtc cagggtatgc
4621 agacgacgca tggcatccgc cataatgctc actttttctg ccggcgccag atggctagac
4681 agcagatcct gacccggcac ttcgcccagc agcagccaat cacggcccgc ttcggtcacc
4741 acatccagca ccgccgcaca cggaacaccg gtggtggcca gccagctcag acgcgccgct
4801 tcatcctgca gctcgttcag cgcaccgctc agatcggttt tcacaaacag caccggacga
4861 ccctgcgcgc tcagacgaaa caccgccgca tcagagcagc caatggtctg ctgcgcccaa
4921 tcatagccaa acagacgttc cacccacgct gccgggctac ccgcatgcag gccatcctgt
4981 tcaatcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
5041 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
5101 ccccgaaaag tgccac
//